Premium
Detoxification of the macrolide toxin brefeldin A by Bacillus subtilis
Author(s) -
Kneusel Richard E.,
Matern Ulrich,
Wray Victor,
Klöppel Klauss-Dieter
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81450-3
Subject(s) - detoxification (alternative medicine) , brefeldin a , bacillus subtilis , toxin , chemistry , microbiology and biotechnology , pharmacology , biology , biochemistry , bacteria , medicine , alternative medicine , pathology , genetics , cell , golgi apparatus
The macrolide brefeldin toxin A is a determinant of Alternaria leaf blight disease in safflower, which causes severe economic losses worldwide. Soilborne bacteria, classified as Bacillus subtilis spp., were isolated and shown to readily metabolize brefeldin A in laboratory culture to one major product. This product was identified by high resolution 2D 1 H NMR and FAB mass spectroscopies as the acid resulting from hydrolysis of the macrolide ring in brefeldin A. In contrast to brefeldin A, the acid completely lacked phytotoxic activity in the standard leaf bioassay. Detoxification of brefeldin A by the lactonase activity from Bacillus subtilis may be exploited in the future to introduce resistance to Alternaria leaf blight in sufflower.