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Bidirectional promoter activity of the 5′ flanking region of the mouse thymidine kinase gene
Author(s) -
Weichslbraun Irene,
Ogris Egon,
Wintersberger Erhard
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81436-r
Subject(s) - chloramphenicol acetyltransferase , thymidine kinase , microbiology and biotechnology , gene , promoter , reporter gene , biology , 5' flanking region , coding region , promoter activity , start codon , gene expression , genetics , messenger rna , virus , herpes simplex virus
The 5′ flanking region of the gene coding for cytoplasmic thymidine kinase (TK) in the mouse (a total of 490 bp upstream of the initiation codon) was tested for promoter activity using the chloramphenicol acetyltransferase gene as reporter. It was found that the region can be devided into two parts, one of which carries promoter activity in the direction of TK, whereas the 5′‐half has promoter activity in the opossite direction. A fragment of 140 bp was sufficient for growth dependent promoter activity in the direction of TK, although about 100 bp further upstream, enhanced the activity. Expressions from the divergent promoter was independent of cell growth.