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Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation
Author(s) -
Dombrádi Viktor,
Axton J.Myles,
Barker Hazel M.,
Cohen Patricia T.W.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81434-p
Subject(s) - mitosis , mutant , biology , premature chromosome condensation , interphase , gene , locus (genetics) , microbiology and biotechnology , genetics , protein phosphatase 1 , phosphatase , chromosome , phosphorylation
A Drosophila gene encoding a protein phosphatase 1(PPI) has been sequenced, and lethal mutations in this locus (87B) analysed. Two mutants ( ck19 e211 and ck19 hs46 ), which disrupt mitosis, lack the 87B isoenzyme and express only ∼20% of wild type PPI activity. The Promoter region of the gene is deleted in the ck19 e11 mutant. A third mutant ( ck19 e078 ), which shows suppression of position effect variegation, but has little effect on mitosis, possesses ∼35% of wild type PPI activity. The results indicate that the PPI 87B isoenzyme is involved in regulation of chromosome condensation at interphase as well as mitosis.