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Formation of a cytosolic ganglioside‐protein complex following administration of photoreactive ganglioside GM1 to human fibroblasts in culture
Author(s) -
Chigorno Vanna,
Valsecchi Manuela,
Acquotti Domenico,
Sonnino Sandro,
Tettamanti Guido
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81406-e
Subject(s) - ganglioside , cytosol , chemistry , biochemistry , enzyme
A GM1 ganglioside derivative bearing a photoreactive nitrophenyl azide group and tritium labeled at the acetyl group of N ‐acetylneuraminic acid, has been administered to cultured human fibroblasts. With photolabeling experiments we found that a portion of the ganglioside in the cell cytosol was associated with a soluble protein of about 30 kDa.