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Interleukin‐1β induces synthesis and secretion of interleukin‐6 in human chondrocytes
Author(s) -
Bender S.,
Haubeck H.-D.,
Van de Leur E.,
Dufhues G.,
Schiel X.,
Lauwerijns J.,
Greiling H.,
Heinrich P.C.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81404-c
Subject(s) - secretion , interleukin , interleukin 1β , synovial fluid , osteoarthritis , in vivo , microbiology and biotechnology , chemistry , endocrinology , interleukin 4 , medicine , western blot , biology , cytokine , biochemistry , pathology , gene , alternative medicine
Increased concentrations of interleukin‐6 (IL‐6) have been found in the synovial fluid of patients with osteoarthritis, rheumatoid arthritis and crystal‐related joint deseases. It is therefore of great interest to identify the cells responsible for the production of IL‐6, and to investigate whether IL‐6 plays a role in the pathogenesisof degenerative or inflammatory joint diseases. Here we show that human interleukin‐1β (IL‐1β) induces IL‐6 synthesis and secretion in differentiated human chondrocytes. In organ cultures resembling closely the in vivo system 10 6 chondrocytes incubated with 100 units of interleukin‐1β per ml of medium led to the release of 6 × 10 3 units of IL‐6 within 24 h. Chondrocytes cultured in agarose or as monolayers similary incubated with IL‐1β produced even higher amounts of IL‐6: 70 × 10 3 units per 10 6 cells within 24 h. The induction of IL‐6 synthesis by IL‐1β was also shown at the mRNA level. IL‐6 secreted by stimulated chondrocytes showed heterogeneity upon Western blot analysis.