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Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P‐450 in rat adrenal cortex
Author(s) -
Imai Michiyo,
Shimada Hideo,
Okada Yukiko,
Matsushima-Hibiya Yuko,
Ogishima Tadashi,
Ishimura Yuzuru
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81398-8
Subject(s) - aldosterone synthase , complementary dna , peptide sequence , cytochrome , microbiology and biotechnology , biology , biochemistry , cdna library , amino acid , adrenal cortex , steroid 11 beta hydroxylase , molecular cloning , enzyme , gene , steroid , endocrinology , renin–angiotensin system , hormone , blood pressure
Using an oligonucleotide probe designed on the basis of the N‐terminal amino acid sequence of purified rat aldosterone synthase cytochrome P‐450 [(1989) J. Biol. Chem. 264 10935] we have isolated from rat adrenal cDNA library a 2687 base pair cDNA that encodes a protein of 500 amino acid residues. The deduced amino acid sequence contained the regions well conserved among all cytochrome P‐450s sequenced to date, and also a portion (residues 25–44) which was identical to the N‐terminal peptide sequence of rat aldosterone synthase cytochrome P‐450. These results indicate that the cDNA encodes a precursor form of rat aldosterone synthase cytochrome P‐450.