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Biosynthesis of acridone alkaloids formation of rutacridone by cell‐free extracts of Ruta graveolens cell suspension cultures
Author(s) -
Maier Walter,
Schumann Brigitte,
Gröger Detlef
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81395-5
Subject(s) - acridone , ruta graveolens , chemistry , suspension culture , biosynthesis , microsome , biochemistry , stereochemistry , cell culture , chromatography , enzyme , biology , botany , genetics
Microsomes prepared either by ultracentrifugation or MgCl 2 precipitation from cultured Ruta graveolens cells catalyze the condensation of 1,3‐dihydroxy‐ N ‐methylacridone and isopentenylpyrophosphate or dimethylallylpyrophosphate. In the presence of NADPH and oxygen rutacridone was identified as reaction product. By omission of NADPH glycocitrine‐II is accumulated. The results suggest that at first a prenylated acridone is formed which in turn is cyclized giving the dihydrofuran part of rutacridone.