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The importance of the C‐terminal amide structure of rat pancreastatin to inhibit pancreatic exocrine secretion
Author(s) -
Miyasaka Kyoko,
Funakoshi Akihiro,
Kitani Kenichi,
Tamamura Hirokazu,
Fujii Nobutaka,
Funakoshi Susumu
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81392-2
Subject(s) - secretion , exocrine pancreas , chemistry , exocrine secretion , terminal (telecommunication) , biochemistry , endocrinology , medicine , pancreas , biology , computer science , telecommunications
A C‐terminal fragment of rat pancreastatin, a 26 residue peptide amide and a fragment without a C‐terminal amide were synthesized by Fmoc‐based solid phase methods and their biological activities were compared. The rat C‐terminal fragment inhibited pancreatic exocrine secretions produced by the intravenous injection of 2‐deoxy‐D‐glucose (a central vagal nerve stimulation), whereas the fragment without a C‐terminal amide showed no effect on pancreas. These results indicate that the C‐terminal amide of this peptide is necessary to reveal its biological activity.