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Inhibition of poly(ADP‐ribose) synthetase by unsaturated fatty acids, vitamins and vitamin‐like substances
Author(s) -
Banasik Marek,
Komura Hajime,
Ueda Kunihiro
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81378-2
Subject(s) - chemistry , biochemistry , ribose , vitamin , food science , enzyme
Various vitamins and vitamin‐like substances inhibited the activity of poly(ADP‐ribose) synthetase in vitro. The most potent were essential fatty acids, i.e. arachidonic acid, linoleic acid, and linolenic acid; their 50% inhibitory concentrations (IC 50 ) were 44–110 μM, indicating a higher potency than nicotinamide, a well‐known vitamin inhibitor (IC 50 = 210 μM). Vitamins K 3 , K 1 , and retinal were the next strongest inhibitors, followed by α‐lipoic acid, coenzyme Q 10 , and pyridoxal 5‐phosphate. Nicotinamide and vitamin K 3 , exhibited mixed‐type inhibition with respect to NAD + ,while arachidonic acid exhibited dual inhibitions, competitive at 50 μM and mixed‐type at 100 μM.