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Intracellular immunization
Author(s) -
Thomas Werge,
Silvia Biocca,
Antonino Cattaneo
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81362-r
Subject(s) - intracellular , antibody , immunization , monoclonal antibody , antigen , biology , microbiology and biotechnology , chemistry , immunology
Following the demonstration that intracellular expression of antibodies ('intracellular immunization') may be utilized to engineer new traits in mammalian cells [1], we undertook experiments to perturb the function of p21 ras proteins, by engineering the intracellular expression of the anti‐p21 ras antibody Y13‐259 [2]. The variable regions of this antibody have been cloned and, after verifying their antigen binding activity, expressed in general purpose vectors for the intracellular expression of antibodies. The results confirmed that the cloned antibody has been efficiently expressed both in the secretory and the intracellular forms. Thus. intracellular immunization of mammalian cells against P21 ras , or any other antigen for which a monoclonal antibody is available, can now be performed.