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Induction of type II iodothyronine 5'‐deiodmase and mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture
Author(s) -
Josef Houšteˇk,
Stanislav Pavelka,
M Baudysová,
Jan Kopecký
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81360-z
Subject(s) - uncoupling protein , receptor , intracellular , stimulation , medicine , endocrinology , iodothyronine deiodinase , incubation , mitochondrion , chemistry , cell culture , biology , deiodinase , biochemistry , brown adipose tissue , triiodothyronine , adipose tissue , thyroid , genetics
Brown adipocytes differentiated in primary cell culture were found to contain a type II iodothyronine 5'‐deiodinase (5'D), Incubation of confluent cells with norepinephrine or dibutyryl‐cAMP caused up to 17‐fold increase in 5'D activity with a maximum after 8 h. Activation of 5'D required mRNA and protein synthesis and was accompanied by parallel, up to 5.8‐fold increase in the amount of mitochondrial uncoupling protein with a maximum after 24 h. Analysis ofadrenergic stimulation of 5'D suggested predominant involvement of the β‐receptors and increased intracellular cAMP levels, while the contribution of x 1 ,‐receptors was small.