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Production of lipocortin‐like proteins by cultured human tracheal submucosal gland cells
Author(s) -
Jacky Jacquot,
Florence Dupuit,
H. Elbtaouri,
Jocelyne Hinnrasky,
F. Antonicelli,
Bernard Haye,
Édith Puchelle
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81347-q
Subject(s) - annexin a1 , polyclonal antibodies , immunofluorescence , annexin , microbiology and biotechnology , submucosal glands , cell culture , antibody , biology , annexin a2 , chemistry , biochemistry , cell , secretion , immunology , genetics
Evidence is obtained for the presence of lipocortin‐like proteins in human tracheal gland cells in culture. Using polyclonal antibodies to lipocortin I. indirect immunofluorescence studies demonstrate that lipocortin I is mainly confined to the tracheal gland cell surface. From cell membranes, four Ca 2+ ‐dependent proteins (35, 40, 45 and 67 kDa) were identified as lipocortin related proteins by using immunoblotting and fluorography following [ 35 S]methionine metabolic labeling experiments. A strong immunoreactivity for the 35 kDa protein was observed. In addition, lipocortinlike proteins with apparent M r 33, 35, 37 and 67 kDa, respectively, were released in the apical culture medium by trachéal gland cells cultured on microporous membrane of a double chamber culture system.