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Thyroid hormone differentially affects mRNA levels of Ca‐ATPase isozymes of sarcoplasmic reticulum in fast and slow skeletal muscle
Author(s) -
Warner S. Simonides,
Gerard C. van der Linden,
C. van Hardeveld
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81332-i
Subject(s) - endocrinology , medicine , endoplasmic reticulum , euthyroid , atpase , messenger rna , skeletal muscle , chemistry , thyroid , calcium atpase , soleus muscle , isozyme , enzyme , biology , biochemistry , gene
mRNA levels for the type I and type II isoforms of sarcoplasmic reticulum (SR) Ca‐ATPase were determined in soleus (SOL) and extensor digitorum longus (EDL) muscle of euthyroid (normal), hypothyroid, and hyperthyroid rats. Total Ca‐ATPase mRNA content of hyperthyroid muscle was 1.5‐fold (EDL) and 6‐fold (SOL) higher compared to hypothyroid muscle, with corresponding increases in total SR Ca‐ATPase activity. EDL contained only type II Ca‐ATPase mRNA. In SOL type I mRNA was the major form in hypothyroidism (98%), but the type II mRNA content was stimulated 150‐fold by T 3 , accounting for 50% of the Ca‐ATPase mRNA in hyperthyroidism.