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Protonated triplex DNA in E. coli cells as detected by chemical probing
Author(s) -
Petr Karlovský,
Petr Pečínka,
Marie Vojtı́šková,
E. Makaturová,
Emil Paleček
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81324-h
Subject(s) - protonation , in vitro , dna , chemistry , intracellular , biophysics , chemical structure , biochemistry , microbiology and biotechnology , crystallography , biology , organic chemistry , ion
The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from that observed in vitro.