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Sodium‐activated potassium current in mouse diaphragm
Author(s) -
Re Lamberto,
Moretti Vincenzo,
Rossini Luigi,
Giusti Piero
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81266-q
Subject(s) - tetraethylammonium , chemistry , sodium , biophysics , potassium , diaphragm (acoustics) , current (fluid) , sodium channel , patch clamp , tetrodotoxin , voltage clamp , extracellular , membrane potential , cardiac transient outward potassium current , biochemistry , biology , physics , receptor , organic chemistry , acoustics , loudspeaker , electrical engineering , engineering
The mouse diaphragm muscle fiber was studied using the loose patch clamp technique. The voltage gated sodium currents were evoked by step pulses from a holding potential of about −70 mV. Following the activation of the sodium current, a very large and fast outward current was evoked. The sensitivity of this current to 4‐aminopyridine and tetraethylammonium indicates the potassium ion as the possible carrier for the channel. Furthermore, the sensitivity to tetrodotoxin and extracellular sodium demonstrated the sodium dependence of this current.