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Functional involvement of calcium in the homologous up‐regulation of the 1,25‐dihydroxyvitamin D 3 receptor in osteoblast‐like cells
Author(s) -
van Leeuwen J.P.T.M.,
Birkenhäger J.C.,
Buurman C.J.,
Schilte J.P.,
Pols H.A.P.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81258-p
Subject(s) - nitrendipine , egta , calcium , extracellular , verapamil , osteoblast , chemistry , calcium in biology , endocrinology , receptor , intracellular , medicine , microbiology and biotechnology , biochemistry , biology , in vitro , organic chemistry
In several cell types 1,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) causes up‐regulation of its receptor. The present study demonstrates that in the osteoblast‐like cell line UMR 106 this up‐regulation is inhibited by two different calcium channel blockers (nitrendipine, verapamil). Also with chelating extracellular calcium (EGTA) and by inhibition of calcium release from intracellular stores (TMB‐8) comparable results were obtained. These findings indicate that calcium is functionally involved in this cellular response to the steroid hormone 1,25(OH) 2 D 3 . Moreover, data obtained with EGTA show that the 1,25(OH) 2 D 3 receptor level is closely regulated by the extracellular calcium concentration.