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A single cell observation of staurosporine effect on the Ca 2+ signals in rat basophilic leukemia cells
Author(s) -
Teshima Reiko,
Ikebuchi Hideharu,
Terao Tadao,
Miyagawa Takehiko,
Arata Yoji,
Nakanishi Mamoru
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81247-l
Subject(s) - staurosporine , calcium , protein kinase c , cytoplasm , protein kinase inhibitor , basophilic , biophysics , protein kinase a , chemistry , microbiology and biotechnology , fluorescence microscope , kinase , biochemistry , biology , fluorescence , medicine , pathology , quantum mechanics , physics , organic chemistry
A digital imaging fluorescence microscope was used to study the effect of a protein kinase inhibitor staurosporine on the antigen‐dependent calcium signals in an individual rat basophilic leukemia cell (RBL‐2H3). Although dose dependency of staurosporine was different from one cell to another, staurosporine inhibited, at low concentration, the calcium influx from the external medium into RBL‐2H3 cells. At high concentration, however, it inhibited both the removal of calcium ion from internal stores and the calcium influx from the external medium. These results indicated that staurosporine is necessary for the inhibition of the calcium influx from the external medium and that a protein kinase (possibly protein kinase C) is involved in the calcium influx from the external medium into the cytoplasm.