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Effects of dideoxyforskolin on proteoglycan synthesis and structure in embryonic chick chondrocyte cultures
Author(s) -
Hu Lie-Min,
Kemp Stephen F.,
Peng Chun-Fu,
Elders M.Joycelyn,
Smith W.Grady
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81243-h
Subject(s) - proteoglycan , embryonic stem cell , chemistry , microbiology and biotechnology , chondrocyte , andrology , anatomy , biology , biochemistry , medicine , in vitro , gene , extracellular matrix
1,9‐Dideoxyforskolin inhibits proteoglycan synthesis and xyloside‐initiated glycosaminoglycan (GAG) synthesis in chick embryo chondrocytes. Dideoxyforskolin does not affect the length of xyloside‐initiated GAG chains secreted into the medium but chains from the dense proteoglycan secreted into the medium appear slightly longer. Incorporation of labeled serine into the dense proteoglycan and subsequent digestion with Pronase revealed a dramatic decrease in percent of total radioactivity associated with GAG chains in the proteoglyean from cultures treated with forskolin or dideoxyforskolin. These observations suggest that these diterpenes have a specific inhibitory effect on chain initiation reactions and thus may be useful tools in the study of proteoglycan synthesis and processing.