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Expression of the cholecystokinin gene in a human (small‐cell) lung carcinoma cell‐line
Author(s) -
Geijer Thomas,
Folkesson Ronnie,
Rehfeld Jens F.,
Monstein Hans-Jürg
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81227-f
Subject(s) - cholecystokinin , northern blot , cell culture , gastrin , messenger rna , radioimmunoassay , gene expression , microbiology and biotechnology , biology , cell , small cell lung carcinoma , gene , medicine , endocrinology , carcinoma , small cell carcinoma , biochemistry , genetics , receptor , secretion
Expression of the cholecystokinin (CCK), gastrin and enkephalin A genes were studied by Northern blot analysis and a library of sequence‐specific radioimmunoassays in human cell lines. The human small‐cell lung carcinoma line (SCLC) U‐1690 expressed moderate levels of CCK mRNA as compared to the human neuroepithelioma cell line SK‐N‐MC. Neither gastrin nor (pro)enkephalin A mRNAs were detectable in the U‐1690 cell line. In contrast, the SCLC‐line H‐69 expressed Enk A but no CCK mRNA. The radioimmunoassays showed that the CCK mRNA transcript in the SCLC line U‐1690 also is translated, and that preproCCK is processed into bioactive, carboxyamidated CCK peptides. Thus, the human small cell carcinoma cell line U‐1690 is a useful model for studies of cell‐specific CCK gene expression.