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Bile acid N ‐acetylglucosaminides
Author(s) -
Matern H.,
Bolz R.,
Marschall H.-U.,
Sjövall J.,
Matern S.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81223-b
Subject(s) - ursodeoxycholic acid , microsome , biochemistry , enzyme , chemistry , bile acid , kidney , primary (astronomy) , biology , endocrinology , physics , astronomy
Bile acid N ‐acetylglucosaminyltransferase activity has been identified in microsomes from human liver and kidney. In both organs the transferases required UDP‐ N ‐aeetylglucosamine as sugar donor and were mainly active towards ursodeoxycholic acid. Minor activities were observed towards amidated ursodeoxycholic, hyodeoxycholic and β‐muricholic acids. No N ‐acetylglucosaminidation was detectable with the major primary and secondary bile acids suggesting a specific requirement of the enzymes for bile acids containing 7β‐ or 6α‐hydroxyl groups. Kinetic parameters and other catalytic properties of liver and kidney microsomal N ‐acetylglucosaminyltransferase activities towards ursodeoxycholic acid are described.