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Efficient aminoacylation of a yeast tRNA Asp transcript with a 5' extension
Author(s) -
Perret Véronique,
Florentz Catherine,
Giegé Richard
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81221-9
Subject(s) - aminoacylation , transfer rna , yeast , genetics , extension (predicate logic) , biology , chemistry , rna , gene , programming language , computer science
A yeast aspartic acid tRNA with a 5' extension of 14 nucleotides was obtained by in vitro transcription with T7 DNA dependent RNA polymerase. This transcript, called extended tRNA Asp transcript, retains its aspartylation capacity with the same K m and only three times reduced k cal values as compared to those measured for canonical tRNA Asp . This result indicates that the 5' extension of the amino acid acceptor stem of tRNA Asp does not interfere with recognition by aspartyl‐tRNA synthetase. However, in contrast to the wild‐type tRNA Asp transcript, the 5' extended molecule presents a reduced capacity to be mischarged by arginyl‐tRNA synthetase, suggesting the existence of different structural requirements in aspartyland arginyl‐tRNA synthetases for tRNA Asp recognition.