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The α 2 B adrenergic receptor of undifferentiated neuroblastoma × glioma hybrid NG108‐15 cells, interacts directly with the guanine nucleotide binding protein, G i 2
Author(s) -
McClue Steven J.,
Milligan Graeme
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81209-7
Subject(s) - yohimbine , gtp' , guanine , microbiology and biotechnology , neuroblastoma , g protein , chemistry , antiserum , receptor , nucleotide , g alpha subunit , antibody , alpha (finance) , protein subunit , cell culture , biochemistry , biology , antagonist , gene , medicine , enzyme , immunology , genetics , construct validity , nursing , patient satisfaction
In membranes of undifferentiated neuroblastoma × glioma hybrid cell line NG108‐15, the apparent specific binding of [ 3 H]yohimbine measured in the presence of 1 μM noradrenaline, was increased substantially by the presence of the poorly hydrolysed analogue of QTP, guanylyl‐imidodiphosphate (Gpp[NH]p) or by preincubation of membranes with antibodies against the C‐terminal decapeptide of the α subunit of the G‐protein G i 2. Such an effect was not produced by antibodies against the equivalent region of G o α G i 3α or G s α or from non‐immune serum. By contrast, total specific binding of [ 3 H]yohimbine was not modified by co‐incubation with Gpp[NH]p or by preincubation with the antibodies from any of the anti‐G protein antisera. These results demonstrate a direct interaction of the α 2 B adrenergic receptor of NG108‐15 cells with G i 2.