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Action of spermidine, N 1 ‐acetylspermidine, and N 8 acetylspermidine at apurinic sites in DNA
Author(s) -
Haukanes B.I.,
Szajko K.,
Helland D.E.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81200-8
Subject(s) - spermidine , polyamine , spermine , dna , cleavage (geology) , biochemistry , biology , chemistry , stereochemistry , enzyme , paleontology , fracture (geology)
The cleavage efficiency of spermidine and its acetyl derivatives (N 1 ‐acetylspermidine and N 8 ‐acetylspennidine) at apurinic sites in DNA were examined by PAGE‐urea analysis. The three polyamines induced different rates of cleavage when compared at 1 mM concentrations. The order of effectiveness were: spermidine < N 8 ‐acetylspermidine < N 1 ‐acetylspermidine. Thus a decrease in efficiency was observed when the first order aminogroups of spermidine were blocked. The N‐ 8 amino‐group of spermidine was less effective in inducing cleavage at AP‐sites than the N 1 ‐amino‐group. Among several proposed models ofpolyamine‐DNA interactions, our results can best be explained by the model postulated by Liquori et al. [6].