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Immunochemical detection of photoaffinity‐labelled capsaicin‐binding proteins from sensory neurons
Author(s) -
Wood John N.,
Walpole Christopher,
James Iain F.,
Dray Andy,
Coote Paul R.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81198-w
Subject(s) - capsaicin , radioimmunoassay , dorsal root ganglion , chemistry , neurotoxin , sensory neuron , blot , biochemistry , agonist , sensory system , biology , receptor , neuroscience , gene
Capsaicin is a plant neurotoxin which depolarises a subset of mammalian sensory neurons. A photoaffinity probe (4‐azidophenylpropionamide) with capsaicin‐like agonist activity (EC 50 5 μM) has been used to covalently label rat and chick sensory neurons in culture, as well as membrane preparations from both neurons and other tissues. Dorsal root ganglion cell specific capsaicin‐binding proteins, including a major band of apparent molecular mass 58 000, have been identified by means of Western blotting, using a specific anti‐capsaicin antiserum characterised by radioimmunoassay with a large range of capsaicin congeners. Using the same radioimmunoassay, no endogenous capsaicin‐like immunoreactive material in normal or inflamed tissue has, however, been detected.