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Cloning of cDNA and genomic DNA for human cytochrome P‐450 11β
Author(s) -
Kawamoto Takeshi,
Mitsuuchi Yasuhiro,
Toda Katsumi,
Miyahara Kaoru,
Yokoyama Yuichi,
Nakao Kazuwa,
Hosoda Kiminori,
Yamamoto Yasutake,
Imura Hiroo,
Shizuta Yutaka
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81190-y
Subject(s) - complementary dna , cloning (programming) , genomic dna , molecular cloning , microbiology and biotechnology , dna , genetics , genomic library , in vitro recombination , biology , gene , chemistry , base sequence , computer science , programming language
A full‐length cDNA clone encoding steroid 11β‐hydroxylase (P‐450 11β ) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'‐untranslated region and a 576 bp 3'‐untranslated region to which a poly(A) tract is attached. The promoter region of the P‐450 11β gene has also been isolated from a genomic library derived from human pre‐B cells. It contains a TATA box, a putative cAMP‐responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP‐1. A transient expression assay in Y‐1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'‐flanking region of the gene suggests that several cis ‐acting elements participate in transcriptional regulation of human P‐450 11β gene.

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