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Resonance Raman spectra of large pea phytochrome at ambient temperature
Author(s) -
Tokutomi Satoru,
Mizutani Yasuhisa,
Anni Helen,
Kitagawa Teizo
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81189-u
Subject(s) - protonation , excitation , resonance (particle physics) , raman spectroscopy , analytical chemistry (journal) , raman scattering , spectral line , chemistry , population , absorption (acoustics) , absorption spectroscopy , phytochrome , nuclear magnetic resonance , materials science , atomic physics , optics , physics , botany , chromatography , biology , ion , demography , red light , organic chemistry , quantum mechanics , astronomy , sociology , composite material
Resonance Raman (RR) scattering from large pea phytochrome was observed at ambient temperature for the first time by using a micro‐spinning cell and the two color excitation technique. The relative population of the red‐absorbing form (P r ), the far red‐absorbing form (P fr ), and the bleached intermediate (I bl ) under laser illumination was estimated from the absorption spectra. The RR spectrum of P r obtained by the 363.8 nm excitation under the 740.0 nm pumping exhibited a frequency shift between the H 2 O and D 2 O solutions, but those of P fr and I bl obtained by the 406.7 nm excitation under the 632.8 nm pumping did not, indicating a distinct difference in their protonation levels, presumably in the protonation of ring C.