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Solubilization of sarcoplasmic reticulum membranes by sodium dodecylsulphate
Author(s) -
Prado A.,
Muga A.,
Castresana J.,
Goñi F.M.,
Arrondo J.L.R.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81185-q
Subject(s) - endoplasmic reticulum , solubilization , sodium , chemistry , membrane , pulmonary surfactant , vesicle , biophysics , sarcoplasm , denaturation (fissile materials) , atpase , biochemistry , chromatography , nuclear chemistry , enzyme , organic chemistry , biology
In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca 2+ ‐ATPase activity, membrane protein solubilization, and protein conformation have been independently monitored, and correlations among the various parameters have been observed. The thermal denaturation of sarcoplasmic reticulum proteins in the presence of sodium dodecylsulphate has also been characterized spectroscopically.