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Isolation and functional expression of pituitary peptidylglycine α‐amidating enzyme mRNA
Author(s) -
Kato Ichiro,
Yonekura Hideto,
Yamamoto Hiroshi,
Okamoto Hiroshi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81184-p
Subject(s) - enzyme , messenger rna , ribonuclease , microbiology and biotechnology , northern blot , biology , alternative splicing , biochemistry , coding region , pituitary gland , gene , rna , hormone
We demonstrate that, in rat pituitary, peptidylglycine α‐amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain‐coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS‐7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.