Premium
Expression of the Drosophila melanogaster metallothionein genes in yeast
Author(s) -
Silar Philippe,
Wegnez Maurice
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81172-k
Subject(s) - drosophila melanogaster , drosophila (subgenus) , gene , yeast , metallothionein , genetics , biology , microbiology and biotechnology
The metallothionein system in Drosophila melanogaster is composed of two genes, Mto and Mn , that code for distinctly different proteins. In order to compare the properties of Mto and Mtn , we transformed yeast with several fusion plasmids. The Mto and Mtn cDNAs, when placed under the control of CUP1 or PGK promoters, can confer a copper‐resistance phenotype to copper‐hypersensitive cells. Both Mto and Mtn proteins can be characterized in extracts from transformed yeast cells.