Premium
Molecular cloning of the β‐subunit of the Na,K‐ATPase in the brine shrimp, Artemia
Author(s) -
Bhattacharyya Kalyan K.,
Bergstrom Ellen E.,
Hokin Lowell E.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81162-h
Subject(s) - brine shrimp , cloning (programming) , protein subunit , shrimp , biology , fishery , chemistry , biochemistry , gene , computer science , programming language
A cDNA encoding the β‐subunit of the Na,K‐ATPase of brine shrimp ( Artemia ) has been cloned. Its nucleotide sequence and predicted amino acid sequence have been determined. The amino acid sequence shows considerable divergence from that of chicken, dog, human, pig, rat, sheep, Torpedo , and Xenopus . This is not entirely unexpected since brine shrimp is a ‘fast clock’ organism which diverged from the precursor of the vertebrates 0.5–1.0 billion years ago. However, a highly hydrophobic putative transmembrane domain and the carboxy‐terminal domain show considerable conservation. The relatively small degree of conservation in the β‐subunit of Anemia should provide information about the functional significance of this protein.