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2‐Deoxy‐D‐glucose resistant yeast with altered sugar transport activity
Author(s) -
Novak Srdjan,
D'Amore Tony,
Stewart Graham G.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81154-g
Subject(s) - derepression , maltose , yeast , glucose transporter , saccharomyces cerevisiae , biochemistry , sugar , mutant , snf3 , chemistry , transport system , biology , psychological repression , sucrose , gene , gene expression , transport engineering , insulin , engineering , endocrinology
The transport of glucose and maltose in Saccharomyces cerevisiae was observed to occur by both high and low affinity transport systems. A spontaneously isolated 2‐deoxy‐D‐glucose resistant mutant was observed to transport glucose and maltose only by the high affinity transport systems. Associated with this was an increase in the V max values, indicating derepression of the high affinity transport systems. The low affinity transport systems could not be detected. This mutant will be important in examining the repression regulatory and sugar transport mechanisms in yeast.