Premium
Fast atom bombardment mass spectrometry and chemical analysis in determinations of acyl‐blocked protein structures
Author(s) -
Egestad Börje,
Estonius Mats,
Danielsson Olle,
Persson Bengt,
Cederlund Ella,
Kaiser Rudolf,
Holmquist Barton,
Vallee Bert,
Parés Xavier,
Jefferey Jonathan,
Jörnvall Hans
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81152-e
Subject(s) - acetylation , fast atom bombardment , mass spectrometry , chemistry , peptide , alcohol dehydrogenase , enzyme , terminal (telecommunication) , biochemistry , yeast , stereochemistry , chromatography , telecommunications , computer science , gene
Peptide generation and fast atom bombardment mass spectrometry in combination with conventional chemical analysis was used to identify the blocking group and establish the N‐terminal structure of six different proteins at the nanomole level. In this manner, the first terminal structures of three non‐mammalian alcohol dehydrogenases were determined, demonstrating the presence of N‐terminal acetylation in these piscine, amphibian, and avian enzymes. Similarly, two different yeast glucose‐6‐phosphate dehydrogenases and a minor variant of a human alcohol dehydrogenase were found to be acetylated. The exact end location of C‐terminal structures was also established. Together, the analyses permit the definition of terminal regions and blocking groups, thus facilitating the delineation of remaining structures.