Premium
Cloning of the human brain GABA transporter
Author(s) -
Nelson Hannah,
Mandiyan Sreekala,
Nelson Nathan
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81149-i
Subject(s) - complementary dna , neurotransmitter transporter , transporter , open reading frame , gaba transporter , amino acid , transmembrane domain , cloning (programming) , human brain , transmembrane protein , biology , molecular cloning , amino acid transporter , biochemistry , peptide sequence , microbiology and biotechnology , gene , neuroscience , receptor , computer science , programming language
A cDNA clone encoding a transporter for the neurotransmitter γ‐aminobutyric acid in human brain was cloned and sequenced. The cDNA contains an open reading frame encoding a hydrophobic protein of 599 amino acids with a calculated molecular weight of 67022 Da. Hydropathy analysis revealed twelve potential transmembrane segments. The human protein is highly homologous to the protein from rat brain. Northern hybridization demonstrated a ubiquitous distribution of the transporter in various parts of the brain.