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Radiorespirometry evidence for the discrimination between 13 C‐enriched glucose and unlabelled glucose molecules by Paracoccus denitrificans
Author(s) -
Dunstan R.H.,
Rees T.ap,
Whatley F.R.,
Greenaway W.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81148-h
Subject(s) - paracoccus denitrificans , carbohydrate metabolism , biochemistry , chemistry , metabolism , carbon source , carbohydrate , biology , enzyme
Paracoccus denitrificans was grown on either unlabelled glucose, [1‐ 13 C]glucose or [6‐ 13 C]glucose as the sole carbon source for growth. The cells were then incubated with a range of 14 C‐glucose substrates to compare the 14 CO 2 ‐evolution rates between cells grown on the glucose and the 13 C‐labelled glucose. Cells grown on 13 C‐glucose had significantly faster rates of 14 CO 2 ‐evolution than those grown on unlabelled glucose. The % yields of 14 CO 2 , per [1‐ 14 C]‐, [6‐ 14 C]‐ and [U‐ 14 C]glucose supplied were also substantially greater than those measured for cells grown on unlabelled glucose. The data indicated that growth of Paracoccus on 13 C‐enriched glucose substrates resulted in cells with notably different 14 C‐glucose oxidation metabolism compared to that observed in cells grown on unlabelled glucose.