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Modulation of calcium mobilization by guanosine 5'‐ O ‐(2‐thiodiphosphate) in Xenopus oocytes
Author(s) -
Sealfbn Stuart C.,
Mundamattom Shirly,
Gillo Boaz
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81138-e
Subject(s) - calcium , xenopus , guanosine , biophysics , voltage clamp , chemistry , intracellular , calcium in biology , endocrinology , biochemistry , membrane potential , biology , organic chemistry , gene
We investigated the effect of intracellular loading of the non‐hydrolyzable guanosine 5'‐diphosphate analogue GDPßS on calcium mobilization by IP 3 and on calcium influx in Xenopus oocytes. Assayed by two electrode voltage‐clamp recording, GDPßS‐loaded oocytes demonstrated a marked augmentation of the fast component of the response to IP 3 injection, an attenuation of the slow component and an increase in membrane calcium permeability. These effects on calcium mobilization suggest that gdpβs may facilitate calcium translocation both across the plasma membrane and between different intracellular calcium pools.