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Mutual dependence of Na,K‐ATPase α‐ and β‐subunits for correct posttranslational processing and intracellular transport
Author(s) -
Ackermann U.,
Geering K.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81130-g
Subject(s) - protein subunit , xenopus , intracellular , chemistry , atpase , biophysics , folding (dsp implementation) , microbiology and biotechnology , intracellular transport , biochemistry , biology , enzyme , gene , electrical engineering , engineering
In this study, we have followed the fate of newly synthesized α‐ and β‐subunits of Na,K‐ATPase in Xenopus oocytes injected with α and/or β cRNA to examine whether assembly of the two subunits is needed for a correct folding and/or for intracellular transport of Na,K‐ATPase. Our data indicate that (1) assembly of α‐ and β‐subunits occurs at the level of the ER, (2) β‐subunits are needed for the newly synthesized α‐subunit to adopt a stable configuration and (3) α‐ and β‐subunits mutually depend on each other to be transported out of the ER.