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Inhibition of γ‐glutamyl transpeptidase decreases amino acid uptake in human keratinocytes in culture
Author(s) -
Viña Juan R.,
Blay Pilar,
Ramirez Ana,
Castells Antonio,
Viña José
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81125-8
Subject(s) - alanine , in vitro , amino acid , leucine , chemistry , biochemistry , cell culture , biology , genetics
Acivicin inhibits γ‐glutamyl transpeptidase activity in human keratinocytes in culture. Treatment of these cells with acivicin produces a decrease in the uptake of L‐[U‐ 14 C]alanine, 2‐amino‐[1‐ 14 C]‐isobutyrate, L[U‐ 14 C]leucine and l‐aminocyclopentane‐l‐[ 14 C]carboxylate. D‐[U‐ 14 C]glucose uptake is not affected by the presence of acivicin. These results support, for the first time in vitro, the hypothesis that the γ‐glutarml cycle may be involved in amino acid uptake by human cells.