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New model of tertiary structure of plant 5S rRNA is confirmed by digestions with α‐sarcin
Author(s) -
Barciszewska Miroslawa Z.,
Lorenz Siegfried,
Joachimiak Andrzej,
Ulbrich Norbert,
Erdmann Volker A.,
Barciszewski Jan
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81124-7
Subject(s) - ribosomal rna , biology , protein tertiary structure , chemistry , biochemistry , gene
The cytotoxin α‐sarcin was employed to test the model of secondary and tertiary structures of plant 5S rRNAs, which we recently proposed [(1990) Int. J. Biol. Macromol. (in press)]. α‐Sarcin is a novel ribonuclease that hydrolyzes phosphodiester bonds adjacent to purines in nucleic acids [2–4]. The digestion pattern obtained for lupin and wheat germ 5S rRNAs strongly suggests the existence of tertiary interactions between residues C34, C35, C36, A37 and G85, G86, 087, U88 as previously proposed. The results on the secondary structure of plant 5S rRNA are in line with a previously proposed model [5]