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Production of enkephalin in tobacco protoplasts using tobacco mosaic virus RNA vector
Author(s) -
Takamatsu Nobuhiko,
Watanabe Yuichiro,
Yanagi Hideki,
Meshi Tetsuo,
Shiba Tadayoshi,
Okada Yoshimi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81121-4
Subject(s) - tobacco mosaic virus , rna , transcription (linguistics) , gene , biology , microbiology and biotechnology , virology , virus , chemistry , biochemistry , linguistics , philosophy
To examine the validity of the strategy to express a foreign gene as a fusion protein with the coat protein (CP) of tobacco mosaic virus (TMV), we have constructed ENK RNA by using an in vitro transcription system of TMV RNA. ENK RNA differs from TMV RNA only in that ENK RNA carries an additional sequence coding for Leu‐enkephalin (Tyr‐Gly‐Gly‐Phe‐Leu) (Enk) with a preceding in‐frame methionine just before the termination codon of CP gene. In protoplasts inoculated with ENK RNA, CP+Enk fusion protein accumulated as the major protein.