Premium
The influence of oligonucleotide‐effector on the selectivity of sequence specific modification of 16 S rRNA
Author(s) -
Zenkova M.A.,
Fedorova O.S.,
Levina A.S.,
Mamaev S.V.,
Karpova G.G.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81110-a
Subject(s) - oligonucleotide , effector , reagent , duplex (building) , chemistry , selectivity , nucleotide , chemical modification , ribosomal rna , biochemistry , dna , gene , organic chemistry , catalysis
The influence of duplex stabilizing oligonucleotide‐effector (oligonucleotide, carrying N ‐(2‐hydroxyethyl)phenazinium residues on both ends), on selectivity of site‐directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constant of cooperative binding of the reagent and the oligonucleotide‐effector with 16 S rRNA was determined. The accuracy of modification was shown to double in the presence of 50 μM effector at 5 μM concentration of the reagent.