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The specific inhibitor of protein kinase C, 1‐(5‐isoquinolinylsulfonyl)‐2‐methylpiperazine (H7), induces morphological change and cell differentiation of human neural crest‐derived cell lineages
Author(s) -
Parodi Maria Teresa,
Varesio Luigi,
Tonini Gian Paolo
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81104-v
Subject(s) - neurite , neural crest , neuroblast , protein kinase c , microbiology and biotechnology , cellular differentiation , biology , cell culture , neuroblastoma , cell growth , protein kinase a , cell , cell type , kinase , embryo , biochemistry , in vitro , neurogenesis , genetics , gene
It has been proved that inhibition of protein kinase C by 1‐(5‐isoquinolinylsulfonyl)‐1‐methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast‐like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in both N and I type morphology treating the cells with 7, 14 or 28 μM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.