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Immunologically cross‐reactive and redox‐competent cytochrome b 6 / f ‐complexes in the chlorophyll‐free plasma membrane of cyanobacteria
Author(s) -
Kraushaar Harald,
Hager Silvia,
Wastyn Marnik,
Peschek Günter A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81091-2
Subject(s) - cyanobacteria , redox , cytochrome , chemistry , chlorophyll , chlorophyll a , biochemistry , membrane , biology , enzyme , inorganic chemistry , organic chemistry , bacteria , genetics
Plasma and thylakoid membranes were separated and purified from cell‐free extracts of the cyanobacteria Anacystis nidulans, Synechocystis 6714, Anabaena variabilis and Nostoc sp. strain Mac. Immunoblots of the membrane proteins using antisera raised against subunits I–IV of the chloroplast b 6 / f ‐complex gave evidence for the presence of a homologous complex in both plasma and thylakoid membranes from the four species of cyanobacteria investigated. Both plasma and thylakoid membranes catalyzed the electron transfer from (exogenous) plastoquinol‐9 and NADH to horse heart ferricytochrome c . However, while with plasma membranes these reactions were severely inhibited by low concentrations of antimycin A and rotenone, respectively, the inhibitors were without major effect on thylakoid membranes. The results will be discussed in terms of a possible similarity (analogy and/or homology?) of cyanobacterial plasma membranes to the inner mitochondrial membrane.