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Regulation of cytochrome P‐450 CYPIA1 gene expression and protooncogene expression by growth factors in primary hepatocytes
Author(s) -
Höhne Martin,
Becker-Rabbenstein Volker,
Kahl Georg F.,
Taniguchi Hisaaki
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81089-7
Subject(s) - biology , epidermal growth factor , transforming growth factor , tgf alpha , growth factor , gene expression , microbiology and biotechnology , gene , cell growth , hepatocyte nuclear factor 4 , receptor , genetics , nuclear receptor , transcription factor
The effect of growth factors on the cytochrome P‐450 (CYPIA1) gene expression was studied in primary mouse hepatocytes. Of the three growth factors used, i.e. epidermal growth factor (EGF), transforming growth factor α (TGFα) and insulin, only EGF or TGFα completely blocked CYPIA1 expression in the presence of the CYPIA1 inducer 3‐methylcholanthrene (3‐MC). This repression was not linked to cell cycle progression of the hepatocyte because insulin was active to induce ‘early immediate genes’ and DNA replication as well as EGF/TGFα but failed to suppress CYPIA1 expression. A specific EGF/TGFα receptor‐mediated function may repress CYPIA1 gene expression and contribute to the acquisition of a xenobiotic drug resistance phenotype.