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Glucose‐stimulated efflux of indo‐1 from pancreatic β‐cells is reduced by probenecid
Author(s) -
Arkhammar Per,
Nilsson Thomas,
Berggren Per-Olof
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81079-4
Subject(s) - efflux , probenecid , chemistry , endocrinology , medicine , stimulation , basal (medicine) , intracellular , insulin , biochemistry , pharmacology , biology
Indo‐1 loaded pancreatic β‐cells, isolated from obese hyperglycaemic mice, were studied with respect to cytoplasmic free Ca 2+ concentration ([Ca 2+ ] i ), efflux of indicator and insulin release. In the absence of glucose there was a continuous efflux of indo‐1 which increased upon stimulation with 20 mM of the sugar. The anion exchange inhibitor probenecid reduced both basal efflux of indo‐1 and prevented that promoted by glucose. Measurements of [Ca 2+ ] i and insulin release revealed similar results as previously reported with quin‐2 and fura‐2. Furthermore, probenecid did not influence the [Ca 2+ ] i responses. It is thus possible to reduce efflux of indo‐1 probenecid and thereby improve the measurements of [Ca 2+ ] i in pancreatic β‐cells.