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Changes of fluorescence anisotropy in plasma membrane of human polymorphonuclear leukocytes during the respiratory burst phenomenon
Author(s) -
Fiorini Rosamaria,
Curatola Giovanna,
Bertoli Enrico,
Giorgi Pier Luigi,
Kantar Ahmad
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81065-v
Subject(s) - respiratory burst , fluorescence anisotropy , respiratory system , fluorescence , chemistry , biophysics , polymorphonuclear leukocyte , membrane , human plasma , granulocyte , immunology , biology , biochemistry , physics , chromatography , anatomy , in vitro , quantum mechanics
Steady state fluorescence anisotropy ( r s ) of TMA‐DPH was measured to study the effect of respiratory burst activation with PMA, FMLP, and PAF on the physico‐chemical structure of PMNs plasma membrane. Our results show a significant increase in r s during the respiratory burst activation. In the presence of NADPH‐oxidase inhibitor DPI, only PAF induces changes in r s values. This suggests a non‐specific effect of PAF on plasma membrane. Azide, which induces a supranormal release of H 2 O 2 , fails to increase the basal r s value after activation. Moreover, the catalase does not abolish the increase in rr s induced upon activation. This rules out the possibility that changes of r s during the respiratory burst activation are attributed mainly to H 2 O 2 release. We conclude that multiple processes accompanying the respiratory burst activation are responsible for the changes in the physico‐chemical properties of PMNs plasma membrane.