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Neurospora crassa cDNA clones coding for a new member of the ras protein family
Author(s) -
Altschuler Daniel L.,
Muro Andres,
Schijman Alejandro,
Almonacid Fernando Bravo,
Torres Hector N.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81061-r
Subject(s) - neurospora crassa , family member , complementary dna , neurospora , genetics , protein family , biology , coding region , coding (social sciences) , microbiology and biotechnology , chemistry , gene , genealogy , history , statistics , mathematics , mutant
A new member of the ras gene family was characterized from Neurospora crassa cDNA libraries. The clone designated NC‐ras codes for a polypeptide containing 213 amino acids ( M r 24000). This polypeptide is 84% homologous to the H‐ras‐1 domain comprising the first 80 amino acids and 60% homologous to the next 84 residues. The NC‐ras polypeptide contains all the well‐known sequences involved in the interaction with GTP/GDP, the recognition of the Y13‐259 neutralizing antibody, the ‘effector site’ for interaction with GAP proteins, and the CAAX acylation motif in the COOH‐terminal.