Premium
Long term culture of rat soleus muscle in vitro
Author(s) -
Stace Pamela B.,
Marchington David R.,
Kerbey Alan L.,
Randle Philip J.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81058-v
Subject(s) - medicine , insulin , endocrinology , soleus muscle , adenosine deaminase , glycogen synthase , glycogen , in vitro , chemistry , denervation , basal (medicine) , adenosine triphosphate , creatine , adenosine , biology , biochemistry , skeletal muscle
Rat soleus muscle strips cultured for 24 h in medium 199 were well preserved in terms of electron microscopy; ATP and creatine phosphate concentrations; rates of glucose utilization, glycogen and protein synthesis, and effects of insulin thereon. Culture led to modest changes in fluid spaces and intracellular (K + ); increased basal glucose utilization up to two‐fold; had no effect on the maximum response to insulin; and had no effect on sensitivity to insulin except in the presence of adenosine deaminase. Thus in vitro neither denervation nor absence of insulin had any marked effects in 24 h to decrease responses to insulin.