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Complementation of the protein transport defect of an Escherichia coli sec Y mutant ( sec Y24 ) by Bacillus subtilis sec Y homologue
Author(s) -
Nakamura Kouji,
Takamatsu Hiromu,
Akiyama Yoshinori,
Ito Koreaki,
Yamane Kunio
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81054-r
Subject(s) - bacillus subtilis , mutant , complementation , escherichia coli , microbiology and biotechnology , biology , homology (biology) , gene product , gene , chemistry , biochemistry , bacteria , genetics , gene expression
Bacillus subtilis SecY homologue shares 41.3% homology with that of E. coli and remarkably higher homologous regions (more than 80%) are present in the four cytoplasmic regions [(1990) J. Biochem. 107, 603‐607]. Based on the formation of the mature form of OmpA in E. coli , we have shown that the protein transport defect of the E. coli sec Y mutant ( secY24 ) is complemented by the gene product from the B. subtilis sec Y homologue, which is expressed under the lac promoter control. However, B. subtilis SecY could not restore growth of the E. coli mutant at non‐permissive temperature.