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A common motif of two adjacent phosphoserines in bovine, rabbit and human cardiac troponin I
Author(s) -
Mittmann Karin,
Jaquet Kornelia,
Heilmeyer Ludwig M.G.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81046-q
Subject(s) - phosphoserine , troponin t , rabbit (cipher) , biochemistry , troponin i , chemistry , troponin , microbiology and biotechnology , biology , enzyme , medicine , statistics , mathematics , serine , myocardial infarction
From rabbit and human cardiac troponin I N‐terminal mono and bisphosphorylated peptides were isolated which were obtained from Lys‐C proteinase digests. Two adjacent phosphoserine residues could be localized in each phosphopeptide following further tryptic digestion. The previously published sequence of rabbit cardiac troponin I had to be corrected. Two adjacent phosphoserine residues are a common motif in the very similar sequences of bovine, rabbit and human cardiac troponin I. The N‐terminal sequences are: AcADRSGGSTAG DTVPAPPPVR RR ANYRAY ATEPHAK (bovine), AcADESTDA‐AG EARPAPAPVR RR ANYRAY ATEPHAK (rabbit), (Ac,A,D/N,G,S,S,D/N,A,A,R) EPRPAPAPIR RR ‐NYRAY ATEPHAK (human).