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Identification of phosphopeptides by mass spectrometry
Author(s) -
Schroeder Werner,
Covey Thomas,
Hucho Ferdinand
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81044-o
Subject(s) - mass spectrometry , chemistry , serine , chromatography , protein mass spectrometry , tyrosine , protein subunit , phosphorylation , phosphate , peptide , biochemistry , tandem mass spectrometry , gene
Phosphopeptides can be identified by ion spray mass spectrometry. The method was tested with phosphokemptide and with a proteolytic digest of one subunit (δ‐subunit) of the nicotinic acetylcholine receptor. In the latter one peptide containing tyrosine phosphate, one with two serine phosphates, and two different peptides each containing one serine phosphate were unambiguously identified. Thus it is proven that ion spray mass spectrometry can be applied for the localization of phosphorylation sites in a known primary structure.